A sperm study abstract submitted to the virtual The Biochemistry Global Summit (25th IUBMB Congress, 46th FEBS Congress and 15th PABMB Congress) from 9th to 14th July 2022 reported bio-effects to sperm from millimeter waves. Millimeter waves are planned for new 5G networks.
P-04.5-019 The role of seminal plasma polyamines in the biological effects of microwave irradiation on human semen in vitro A. Hilal1 , M. Ploskonos2 , M. Blagonravov1 , S. Syatkin1 , S. Chibisov1 , E. Kharlitskaya1 , I. Eremina1 , V. Kuznetsov1 , Z. Kaitova1 , L. Varekha.1 1 Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia, 2 Astrakhan State Medical University of The Healthcare Ministry of Russian Federation, Astrakhan, Russia
The aim of the present study was to investigate the influence of millimeter-wave electromagnetic (MW) irradiation on normal and pathological human sperm in vitro, and to evaluate a possible role of polyamines (PA) in this process. The stability of sperm membranes, the number of apoptotic gametes, and the content of seminal plasma PA in the ejaculates of fertile and subfertile men were compared before and after short-term MW electromagnetic exposure in vitro. The ejaculate samples were collected from healthy donors [n = 25, age 22-38 years old (y.o.), average age 30.6 1.1 y.o. (mean SEM)] and from subfertile men (n = 78, age 25-48 y.o., average age 34.1 0.8 y.o.) and exposed to MW radiation. The electromagnetic field had a wavelength of 7.1 mm, a frequency of 42.194 GHz and an exposure time of 20 min.
The fragility of sperm membranes was evaluated by their resistance to sodium chloride solution (Milovanov test) and to acetic acid (Joel test). Acrosin activity was assayed spectrophotometrically. Apoptosis was determined by the externalization of phosphatidylserine on the outer side of the sperm membrane and propidium iodide staining. The PA levels were determined by agar gel electrophoretic fractionation.
An increase in the resistance of sperm membranes, a decrease in acrosin activity, a decrease in the number of apoptotic gametes and a decrease in the seminal plasma PA concentrations were found after exposure of the native human sperm to low-intensity MW irradiation. Two types of reactions were revealed for the subfertile samples. The results revealed positive bio-effects of specific microwaves on the human semen and the participation of PA in the realization of these effects.
European Parliament requested a research report “Health Impact of 5G” released in July 2021 concluding that commonly used RFR frequencies (450 to 6000 MHz) are probably carcinogenic for humans and clearly affect male fertility with possible adverse effects on the development of embryos, fetuses and newborns. 5G will increase ambient levels of wireless radiofrequency radiation. Peer-reviewed research has demonstrated a myriad of adverse effects from wireless radiofrequency radiation including increased brain cancer, DNA damage, oxidative stress, immune dysfunction, altered brain development, damaged reproduction, sleep changes, hyperactivity, and memory damage. (RESEARCH HERE)
Thousands of doctors and scientists are calling to halt 5G because of the increase in radiofrequency radiation- a new kind of environmental pollution. (DOCTORS & SCIENTISTS HERE, Read and download letter from Dr. Davis on the health and environmental effects of 5G)
“There is a substantial body of evidence that this technology is harmful to humans and the environment. The 5G millimeter wave is known to heat the eyes, skin, and testes… Of particular concern are the most vulnerable among us — the unborn, children, the infirm, the elderly, and the disabled. It is also expected that populations of bees and birds will drastically decline.” -Dr. Lennart Hardell
According to Belyaev 2019, “the health effects of chronic MMW exposures may be more significant than for any other frequency range.” The abstract states that, “ Various responses to non-thermal microwaves (MW) from mobile communication including adverse health effects related to electrohypersensitivity, cancer risks, neurological effects, and reproductive impacts have been reported while some studies reported no such effects. This presentation provides an overview of the complex dependence of the MW effects on various physical and biological variables, which account for, at least partially, an apparent inconsistence in the published data. Among other variables, dependencies on carrier frequency, polarization, modulation, intermittence, electromagnetic stray fields, genotype, physiological traits, and cell density during exposure were reported. Nowadays, biological and health effects of 5G communication, which will use microwaves of extremely high frequencies (millimeter waves MMW, wavelength 1- 10 mm), are of significant public concern. It follows from available studies that MMW, under specific conditions of exposure at very low intensities below the ICNIRP guidelines, can affect biological systems and human health. Both positive and negative effects were observed in dependence on exposure parameters. In particular, MMW inhibited repair of DNA damage induced by ionizing radiation at specific frequencies and polarizations. To what extend the 5G technology and the Internet of Things will affect the biota and human health is definitely not known. However, based on possible fundamental role of MMW in regulation of homeostasis and almost complete absence of MMW in atmosphere due to effective absorption, which suggests the lack of adaptation to this type of radiation, the health effects of chronic MMW exposures may be more significant than for any other frequency range.”
The study “Human Electromagnetic Field Exposure in 5G at 28 GHz” published in IEEE Consumer Electronics Magazine evaluated human exposure to radiofrequency for three wireless systems -5G, 4G, and 3.9G and found that 5G’s higher frequencies penetrate into the skin very intensely despite the fact that the depth of penetration is more shallow.“The SAR is inversely proportional to the penetration depth, and hence, a shallower penetration occurring in 5G yields a higher absorption.” The authors conclude that “the fact that a high-frequency EMF cannot penetrate deep into human skin does not mean that it is not dangerous.”
“The Human Skin as a Sub-THz Receiver – Does 5G Pose a Danger to It or Not?”, published in Environmental Research, and “The Modeling of the Absorbance of Sub-THz Radiation by Human Skin”, published in IEEE Transactions on Terahertz Science and Technology, are two papers by physicists presenting research that found higher 5G frequencies are intensely absorbed into human sweat ducts (in skin), at a much higher absorption levels than other parts of our skin’s tissues (Betzalel et al., 2017, Betzalel et al., 2018). In an article published in Environmental Research, researchers conclude, “We are raising a warning flag against the unrestricted use of sub-THz technologies for communication, before the possible consequences for public health are explored” (Betzalel, et al., 2018).
A 2020 study published in the journal Environmental Research and Public Health investigated the combined exposure of 3 G mobile systems at 1950 MHz RF and UV found a 24-h RF exposure significantly reduced the MMP-1 enzyme concentration, caused by prior UV exposure. Although they did not find changes in cytokines due to exposure to RF alone or enhancment of the effects of UV radiation, their findings indicate changes after exposure and point to the importance of investigating this and other effects on skin. The authors conclude that, “the investigation of the possible adverse effects on the skin due to the high frequency electromagnetic fields become more and more important before the deployment of 5G mobile systems. Using this new technology, the absorption of exposure to RF in the skin will be enhanced. The skin will be the most important target organ of the RF exposure to 5G. Therefore, our approach of combined (i.e., consecutive) exposure to UV and RF might be important in future research related to 5G and skin.”
A 2021 paper “Millimeter (MM) wave and microwave frequency radiation produce deeply penetrating effects: the biology and the physics”by Martin L Pall PHD reviwes the science on howMMwaves can have highly penetrating effects stating that “in humans, MM-waves have penetrating effects including impacts on the brain, producing EEG changes and other neurological/neuropsychiatric changes, increases in apparent electromagnetic hypersensitivity and produce changes on ulcers and cardiac activity…Specifically, MM-wave electrical fields are almost completely absorbed in the outer 1 mm of the body due to the high dielectric constant of biological aqueous phases. However, the magnetic fields are very highly penetrating. 3. Time-varying magnetic fields have central roles in producing highly penetrating effects. The primary mechanism of EMF action is voltage-gated calcium channel (VGCC) activation with the EMFs acting via their forces on the voltage sensor, rather than by depolarization of the plasma membrane…There are three very important findings here which are rarely recognized in the EMF scientific literature: coherence of electronically generated EMFs; the key role of time-varying magnetic fields in generating highly penetrating effects; the key role of both modulating and pure EMF pulses in greatly increasing very short term high level time-variation of magnetic and electric fields. It is probable that genuine safety guidelines must keep nanosecond timescale-variation of coherent electric and magnetic fields below some maximum level in order to produce genuine safety. These findings have important implications with regard to 5G radiation.”
